Enzyme immobilisation in biocatalysis: why, what and how.

Tools in Biocatalysis Enzyme Immobilisation on Silica and Synthesis of Enantiopure Amines

This thesis presents two techniques in the field of biocatalysis: An enzyme immobilisation method based on the His6-tag for attachment on modified silica oxide beads, and it’s employment in aqueous and organic medium for synthesis applications. The method functions as a one step extraction and immobilisation protocol. An equilibrium displacement system which enables complete conversion in react...

Solid-binding peptides for immobilisation of thermostable enzymes to hydrolyse biomass polysaccharides

BACKGROUND Solid-binding peptides (SBPs) bind strongly to a diverse range of solid materials without the need for any chemical reactions. They have been used mainly for the functionalisation of nanomaterials but little is known about their use for the immobilisation of thermostable enzymes and their feasibility in industrial-scale biocatalysis. RESULTS A silica-binding SBP sequence was fused ...

Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness

BACKGROUND Horseradish Peroxidase (HRP) plays important roles in many biotechnological fields, including diagnostics, biosensors and biocatalysis. Often, it is used in immobilised form. With conventional immobilisation techniques, the enzyme adheres in random orientation: the active site may face the solid phase rather than bulk medium, impeding substrate access and leading to sub-optimal catal...

The Why, Who, What, How, and When of Patient Engagement in Healthcare Organizations: A Response to Recent Commentaries

Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles.

The immobilisation of proteins on to nanoparticles has a number of applications ranging from biocatalysis through to cellular delivery of biopharmaceuticals. Here we describe a phosphopantetheinyl transferase (Sfp)-catalysed method for immobilising proteins bearing a small 12-mer "ybbR" tag on to nanoparticles functionalised with coenzyme A. The Sfp-catalysed immobilisation of proteins on to na...